Protocols & Datasets

In situ hybridization technique

Published protocol for the use of cRNA probes for colorometric in situ hybridization in frozen tissue sections.

Published protocol for the processing and procurement of 3 micron frozen sections.

Double-label in situ hybridization (whole mounts)

Unpublished protocol for visualizing two riboprobes with flourescence (fast red / TSA-FITC).

Müller Glia Transcriptome in Regenerating Retina

Microarray gene profiling of isolated, EGFP+ cells from Tg[gfap:EGFP]mi2002 transgenic fish. Genes whose expression levels changed at 8, 16, 24 and 36 hours after intense light lesion compared with uninjured retina. The isolated cells include Müller glia, injury-induced Müller glial stem cells and retinal progenitors derived from them.

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Last modified 03/06/09