4/9/93

Antibiotics

 

Antibiotic

Stock conc

(mg/ml)

Final conc

(μg/ml)
   

E. coli

(Big Red)

E. coli

LB Plates*

Caulobacter

PYE plates *

Caulobacter

liquid media

Ampicillin

100

100

100

50

5 - 10

Chloramphenicol,

in ethanol

5

20

30

1

  

Gentamycin

2.5

15

20

5

2.5

Kanamycin

50

30

50

20

5

Nalidixic acid,

sodium salt

10

15

20

20

-

Oxytetracycline,

in 70% EtOH

5

12

12.5

2

1

Spectinomycin

10

100

50

50

25

Streptomycin

50

30

30

5

5
           

Rule of thumb for CB in liquid media: Use half the conc. of antibiotic used in solid media.

Notes:

Storage: All antibiotics should be stored at 4° C, Tet should be stored at -20° C.

Tet is also light sensitive. Store stock solutions & plates in the dark.

Prep: All antibiotics should be dissolved in sterile distilled water unless otherwise indicated.

Use oxytetracycline for Caulobacter strains.